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How to Get Pee Out of a Mattress | Remove Stains & Odors

By: Susie
Updated On: August 6, 2025

Discovering urine on your mattress can feel overwhelming, but don't panic. Whether it's from a child's accident, pet mishap, or elderly care situation, you can effectively remove urine from mattress surfaces using simple household ingredients.

The key is acting quickly, fresh urine is much easier to clean than dried stains. With the right approach, you'll have your mattress fresh and odor-free in just a few hours using items like white vinegar, baking soda, and hydrogen peroxide.

This comprehensive guide walks you through proven methods to tackle both fresh accidents and set-in stains. You'll learn how to eliminate urine smell from mattress fibers completely, handle pet accidents with special enzyme cleaners, and prevent future mishaps. We've researched the most effective techniques and compiled them into easy-to-follow steps that actually work.

Contents

Why Quick Action Matters When Cleaning a Urine Soaked Mattress?

Time is your biggest enemy when dealing with urine accidents. The moment urine hits your mattress, it begins soaking deep into the fibers, foam layers, and even the mattress core. This penetration makes stain and odor removal increasingly difficult.

Urine contains uric acid crystals that bind to mattress materials. As these crystals dry, they become harder to dissolve and remove. The ammonia in urine also intensifies over time, creating that unmistakable smell that seems impossible to eliminate.

Acting within the first 10-15 minutes can mean the difference between a simple cleanup and hours of intensive treatment. Fresh urine responds well to basic cleaning solutions, while dried stains may require multiple applications and stronger treatments.

Essential Supplies for Urine Removal

Before you begin cleaning a mattress with urine, gather these essential supplies. Having everything ready prevents delays that allow stains to set deeper.

Basic Cleaning Arsenal:

  • White vinegar (distilled)
  • Baking soda
  • Cold water
  • Spray bottles (2-3)
  • Clean white towels or paper towels
  • Vacuum cleaner with upholstery attachment
  • Rubber gloves

Advanced Stain Fighters:

  • Hydrogen peroxide (3% solution)
  • Liquid dish soap
  • Enzyme cleaner (for pet urine)
  • Essential oils (optional, for scent)
  • Plastic wrap
  • Fan or hair dryer

Keep these supplies in an easily accessible location. Quick access means faster response time when accidents happen.

How to Remove Fresh Urine from Your Mattress?

When you catch an accident immediately, follow these steps to clean urine off a mattress before it sets:

Step 1: Strip and Wash Bedding Immediately

Remove all bedding, including sheets, mattress protectors, and blankets. Get them into the washing machine right away with hot water and a cup of white vinegar added to your regular detergent.

Step 2: Blot, Never Rub, The Wet Area

Using clean, dry towels, gently blot the urine spot. Press down firmly to absorb as much liquid as possible. Replace towels as they become saturated. Avoid rubbing, which pushes urine deeper into mattress layers.

Step 3: Create Your Cleaning Solution

Mix equal parts white vinegar and cold water in a spray bottle. Add 2-3 tablespoons of liquid laundry detergent. This solution neutralizes ammonia and breaks down urine compounds.

Step 4: Saturate the Affected Area

Spray the solution generously over the entire stained area. Don't be shy, you need enough liquid to reach as deep as the urine penetrated. Let it sit for 10-15 minutes.

Step 5: Blot Again

After the solution has had time to work, blot the area again with fresh, dry towels. You're removing both the cleaning solution and the dissolved urine.

Step 6: Apply Baking Soda

Cover the entire wet area with a thick layer of baking soda. This natural deodorizer absorbs remaining moisture and neutralizes odors. Leave it for 8-10 hours or overnight.

Step 7: Vacuum Thoroughly

Once the baking soda is completely dry, vacuum it up using your upholstery attachment. Check for any lingering odor or visible staining. Repeat the process if necessary.

Removing Dried Urine Stains: Advanced Techniques

Dealing with set-in stains requires more intensive methods to remove urine from mattress human accidents that have dried:

Method 1: Enhanced Vinegar Treatment

Create a stronger solution using:

  • 2 cups white vinegar
  • 1 cup cold water
  • 3 tablespoons laundry detergent

Apply enough solution to completely saturate the dried stain. Cover the area with plastic wrap to prevent evaporation. This keeps the solution active longer, breaking down stubborn uric acid crystals.

Let it sit for 24-48 hours, then remove the plastic and allow the mattress to air dry completely. This extended contact time helps dissolve deeply embedded stains.

Method 2: Hydrogen Peroxide Power Clean

For stubborn stains that won't budge, try this powerful mixture:

  • 1 cup hydrogen peroxide (3%)
  • 3 tablespoons baking soda
  • 2-3 drops liquid dish soap

Mix ingredients in a spray bottle, swirling gently (don't shake vigorously). Spray liberally on the stain and let it dry completely. As it dries, you'll see baking soda residue form, this indicates the solution is working.

Vacuum the residue and inspect results. This method works especially well on older, yellowed stains.

How to Deodorize Mattress Urine Smells?

Sometimes stains disappear but odors linger. Here's how to remove urine smell from mattress surfaces completely:

Baking Soda Paste Method

Create a thick paste using:

  • 1 cup baking soda
  • 1/2 cup water
  • 10 drops essential oil (lavender or tea tree)

Spread the paste over the affected area using a spatula or old credit card. Work it into the mattress surface with a soft brush. Let it dry completely (4-6 hours), then vacuum thoroughly.

Deep Deodorizing Treatment

For persistent odors, try this overnight treatment:

  1. Spray the area with straight white vinegar
  2. While still damp, sprinkle baking soda liberally
  3. Cover with a clean towel
  4. Place a heavy book on top to press the treatment into the mattress
  5. Leave for 12-24 hours
  6. Remove towel and vacuum

This compression method forces the deodorizing agents deeper into mattress layers where odors hide.

Special Considerations: How to Remove Pet Urine from Mattress?

Pet urine presents unique challenges due to its higher concentration and different chemical composition. Here's how to tackle these tougher stains:

Understanding Pet Urine Differences

Pet urine contains more proteins and hormones than human urine. Cats, in particular, have extremely concentrated urine with strong territorial markers. Dogs' urine may contain additional fatty acids.

Enzyme Cleaner Protocol

Skip homemade solutions for pet accidents. Enzyme cleaners specifically break down pet urine components:

  • Blot fresh urine with paper towels
  • Apply enzyme cleaner according to label instructions
  • Allow it to sit for the recommended time (usually 10-15 minutes)
  • Blot excess moisture
  • Let the area air dry completely
  • Repeat if necessary

Never use ammonia-based cleaners on pet urine, the smell can attract pets back to the same spot.

Preventing Repeat Accidents

After cleaning, use pet deterrent sprays around your bed area. Consider keeping bedroom doors closed or using waterproof mattress protectors designed for pet owners.

Mattress-Specific Cleaning Considerations

Different mattress types require adjusted cleaning approaches:

Mattress TypeSpecial ConsiderationsDrying Time
Memory FoamUse minimal liquid; foam absorbs deeply24-48 hours
InnerspringCan handle more moisture; ensure springs don't rust12-24 hours
LatexAvoid harsh chemicals; use gentle solutions18-24 hours
HybridFollow guidelines for top layer material24-36 hours

Memory foam mattresses need extra care since they're like sponges. Use spray bottles for controlled application and ensure complete drying to prevent mold growth.

Safety Precautions When Cleaning

Protect yourself and your family while cleaning a urine soaked mattress:

Chemical Safety

Never mix cleaning products. According to the CDC's guidelines on household cleaning, combining bleach with ammonia or acids creates toxic gases. Always use products separately.

Ventilation Requirements

Open windows and use fans during cleaning. Good airflow helps:

  • Disperse cleaning solution fumes
  • Speed drying time
  • Prevent mold growth
  • Reduce chemical exposure

Protective Gear

Wear rubber gloves when handling urine-soaked materials. If you have sensitive skin or allergies, consider wearing a mask while applying cleaning solutions.

Preventing Future Accidents

Prevention saves time, money, and stress. Implement these strategies:

Invest in Quality Protection

A good waterproof mattress protector is your first line of defense. Look for:

  • Full encasement design
  • Breathable waterproof layer
  • Machine washable material
  • Hypoallergenic properties

Establish Bedtime Routines

For children:

  • Limit fluids 2 hours before bed
  • Mandatory bathroom trip before sleep
  • Consider overnight pull-ups during training
  • Use nightlights for easy bathroom access

Pet Management Strategies

  • Regular outdoor bathroom breaks
  • Crate training for puppies
  • Keep litter boxes clean and accessible
  • Address medical issues promptly

When to Consider Professional Cleaning

Sometimes DIY methods aren't enough. Consider professional mattress cleaning when:

  • Multiple cleaning attempts fail
  • Urine has penetrated through to the box spring
  • Mold or mildew develops
  • The mattress is expensive or under warranty
  • Health concerns exist (allergies, asthma)

Professional cleaners use industrial-strength equipment and specialized solutions that penetrate deeper than household methods.

Alternative Solutions and Products

Beyond traditional cleaning methods, consider these options:

Commercial Urine Removers

Several products specifically target urine stains:

  • Nature's Miracle Urine Destroyer
  • Rocco & Roxie Professional Strength
  • Anti Icky Poo Unscented

UV Light Detection

Blacklight flashlights help locate old, invisible urine stains. This ensures you treat all affected areas, not just visible spots.

Ozone Generators

For severe odor issues, ozone generators neutralize smells at the molecular level. Use with caution and follow manufacturer safety guidelines.

Long-Term Mattress Care After Urine Exposure

After successfully cleaning your mattress, maintain its freshness:

Monthly Maintenance

  • Vacuum mattress surface
  • Sprinkle baking soda monthly
  • Rotate mattress every 3 months
  • Check for new stains under good lighting

Annual Deep Cleaning

Even without accidents, yearly deep cleaning extends mattress life. Consider scheduling professional cleaning during seasonal mattress sales if replacement becomes necessary.

Knowing When It's Time to Replace

Despite best efforts, sometimes replacement is the only option. Consider getting a new mattress when:

  • Persistent odors remain after multiple cleanings
  • Staining covers more than 25% of surface
  • Mold or mildew develops
  • The mattress is over 8 years old
  • Structural damage occurs from repeated wetting

Frequently Asked Questions

How long does urine take to dry in a mattress?

Fresh urine can dry within 2-4 hours on the surface but remains wet inside mattress layers for 8-10 hours. Complete drying after cleaning takes 24-48 hours depending on humidity and airflow.

Can I use bleach to clean urine from my mattress?

Never use bleach on mattresses. It damages fabrics, breaks down foam materials, and creates toxic fumes when mixed with ammonia in urine. Stick to vinegar-based solutions for safety.

Why does urine smell worse over time?

Bacteria break down urea in urine, producing increasingly strong ammonia odors. Uric acid crystals also concentrate as moisture evaporates, intensifying the smell.

Will steam cleaning remove urine from mattresses?

Steam cleaning can help but may not reach deep enough for thorough urine removal. The heat can also set stains permanently. Chemical treatments work better for complete elimination.

How often should I replace my mattress protector?

Quality mattress protectors should last 2-3 years with regular washing. Replace immediately if tears or leaks develop.

Can old urine stains make you sick?

While not typically dangerous, old urine can harbor bacteria and trigger allergies. Thorough cleaning eliminates health risks and improves sleep quality.

Conclusion: Fresh, Clean Sleep Awaits

Successfully removing urine from your mattress doesn't require expensive products or professional services. With quick action and the right techniques, you can eliminate urine smell from mattress surfaces and restore freshness.

Remember the golden rules: act fast, use appropriate cleaning solutions, and ensure complete drying. Whether dealing with childhood accidents, pet mishaps, or elderly care situations, these methods provide reliable results.

Invest in prevention through quality mattress protection and maintain regular cleaning routines. Your mattress is a significant investment in your health and comfort, treat it well, and it will provide years of restful sleep.

Don't let accidents discourage you. With this guide's techniques, you're equipped to handle any urine challenge and keep your mattress fresh, clean, and inviting for years to come.

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